Title

Techniques of Preparing Plant Material for Chromatographic Separation and Analysis

Document Type

Article

Publication Date

3-10-2007

Publication Title

Journal of Biochemical and Biophysical Methods

Abstract

This paper discusses preparation techniques of samples of plant material for chromatographic analysis. Individual steps of the procedures used in sample preparation, including sample collection from the environment or from tissue cultures, drying, comminution, homogenization, leaching, extraction, distillation and condensation, analyte enrichment, and obtaining the final extracts for chromatographic analysis are discussed. The techniques most often used for isolation of analytes from homogenized plant material, i.e., Soxhlet extraction, ultrasonic solvent extraction (sonication), accelerated solvent extraction, microwave-assisted extraction, supercritical-fluid extraction, steam distillation, as well as membrane processes are emphasized. Sorptive methods of sample enrichment and removal of interferences, i.e., solid-phase extraction, and solid-phase micro-extraction are also discussed.

Volume

70

Issue

2

First Page

253

Last Page

261

DOI

https://doi.org/10.1016/j.jbbm.2006.09.012

ISSN

0165-022X

Comments

ESSN: 0165-022X

Rights

© 2006 Elsevier B.V.

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