Title

Wheat Germ Acid Phosphatase Activity in High Percentages of Organic Solvents

Document Type

Article

Publication Date

4-20-2020

Publication Title

The FASEB Journal

Abstract

While enzymes canonically operate in aqueous environments, several have been shown to also function in non-aqueous solvents and/or solvent mixes. Phosphatases, for example, have been previously shown to hydrolyze phosphates in mixtures containing modest amounts of certain organic solvents. Here, detecting the fluorescent dephosphorylation product naphthol AS-MX with fluorescence spectroscopy (ex: 388 nm, em: 512 nm) has shown commercial wheat germ acid phosphatase to be active in high amounts (up to ~70% by volume) of 1,4-dioxane, 1,2- dimethoxyethane, 2-methoxyethanol, dimethyl sulfoxide, and acetonitrile when the aqueous component was comprised of Tris buffer (pH 7). These solvent mixtures were also physically characterized to better understand differences in both relative naphthol AS-MX fluorescence and wheat germ acid phosphatase activity across the different solvent mixtures investigated. These results suggest that phosphatase activity can be detected with less water that previously published.

Volume

34

Issue

S1

DOI

10.1096/fasebj.2020.34.s1.09870

ISSN

Print: 0892-6638, Electronic: 1530-6860

Comments

Acknowledgment is made to the Donors of the American Chemical Society Petroleum Research Fund and to the National Science Foundation’s Research Experience for Undergraduates program for partial support of this research.

Rights

© 2021 Federation of American Societies for Experimental Biology (FASEB)

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