Title

Sample preparation procedure for the determination of polycyclic aromatic hydrocarbons in petroleum vacuum residue and bitumen

Document Type

Article

Publication Date

6-7-2011

Publication Title

Analytical and Bioanalytical Chemistry

Abstract

This paper describes a novel method of sample preparation for the determination of trace concentrations of polycyclic aromatic hydrocarbons (PAHs) in high-boiling petroleum products. Limits of quantitation of the investigated PAHs in materials of this type range from tens of nanograms per kilogram to <20 μg/kg. The studies revealed that in order to separate most of interferences from the analytes without a significant loss of PAHs, it is necessary to use size exclusion chromatography as the first step of sample preparation, followed by adsorption using normal-phase liquid chromatography. The use of orthogonal separation procedure described in the paper allows the isolation of only a group of unsubstituted and substituted aromatic hydrocarbons with a specific range of molar mass. The lower the required limit of quantitation of PAHs, the larger is the scale of preparative liquid chromatography in both steps of sample preparation needed. The use of internal standard allows quantitative results to be corrected for the degree of recovery of PAHs during the sample preparation step. Final determination can be carried out using HPLC-FLD, GC-MS, or HPLC-UV-VIS/DAD. The last technique provides a degree of identification through the acquired UV-VIS spectra.

Volume

401

Issue

3

First Page

1059

Last Page

1069

DOI

https://doi.org/10.1007/s00216-011-5134-9

ISSN

1618-2642

Comments

ESSN: 1618-2650

Rights

Springer Nature

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